Cryopreservation of human granulocytes in liquid nitrogen.
نویسندگان
چکیده
منابع مشابه
Cryopreservation of human granulocytes in liquid nitrogen.
Human granulocytes (PMNL) were successfully cryopreserved for up to 14 months. The PMNL (1-2 X 10(7)/ml) were stored in 2-ml ampoules in the gas phase of liquid nitrogen at a temperature between -160 degrees C and -196 degrees C using dimethylsulphoxide (DMSO 10%) as cryoprotectant. Morphology and phagocytic and bactericidal capacity were best preserved by adding fetal calf serum to the freezin...
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Human granulocytes can be cryopreserved with dimethyl sulfoxide (DMSO) at--80 degrees C. However, the percent recovery of functional cells has been unsatisfactory to date. Throughout this study we have been able to prepare relatively pure granulocytes approximately equal to 85%), cryopreserve them with 5%--10% DMSO with and without serum, and store them at--80 degrees C for up to 4 mo. The para...
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Human granulocytes can be cryopreserved with dimethyl sulfoxide (DMSO) at 80’C. However, the percent recovery of functional cells has been unsatisfactory to date. Throughout this study we have been able to prepare relatively pure granulocytes ( 85%). cryopreserve them with 5%-1O% DMSO with and without serum, and store them at -80’C for up to 4 mo. The parameters studied were absolute cell count...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1977
ISSN: 0021-9746
DOI: 10.1136/jcp.30.8.758